High-titer expression of recombinant antibodies by transiently transfected HEK 293-6E cell cultures
نویسندگان
چکیده
منابع مشابه
High-yield production of recombinant antibody fragments in HEK-293 cells using sodium butyrate.
To develop new recombinant monoclonal antibody fragments for therapy and imaging, it is indispensable to have a simple and easy procedure to handle the eukaryotic expression system for production of proteins in high amounts. Gene amplification techniques such as the dehydrofolate reductase (DHFR) system in Chinese hamster ovary cells or the glutamine synthase system in myeloma cells have a coup...
متن کاملThe ethics of HEK 293.
1 Congregation for the Doctrine of the Faith, Donum vitae (February 2, 1987), Introduction, 5. 2 Ibid., I, 1. The topic of embryonic stem cells is very much in the forefront of public interest. The moral stance on obtaining stem cells from embryos is clear: The Church has always taught that all human life from the very beginning must be respected and protected. If human embryos are killed to ob...
متن کاملComparison of neuronal and endothelial isoforms of nitric oxide synthase in stably transfected HEK 293 cells.
The neuronal and endothelial isoforms of nitric oxide (NO) synthase (nNOS and eNOS, respectively) both catalyze the production of NO but are regulated differently. Stably transfected HEK 293 cell lines containing nNOS, eNOS, and a soluble mutant of eNOS were therefore established to compare their activity in a common cellular environment. NOS activity was determined by measuring L-[3H]citrullin...
متن کاملInteraction of neuronal nitric oxide synthase with alpha1-adrenergic receptor subtypes in transfected HEK-293 cells
BACKGROUND The C-terminal four amino acids (GEEV) of human alpha1A-adrenergic receptors (ARs) have been reported to interact with the PDZ domain of neuronal nitric oxide synthase (nNOS) in a yeast two-hybrid system. The other two alpha1-AR subtypes have no sequence homology in this region, raising the possibility of subtype-specific protein-protein interactions. RESULTS We used co-immunopreci...
متن کاملEbola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression...
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ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2015
ISSN: 1753-6561
DOI: 10.1186/1753-6561-9-s9-p40